Fig 2. Hyd is required for the full activation of IMD response.

(A) Quantitative RT-PCR of Attacin-A, Attacin-C, Cecropin-A1, Attacin-D, Pgrp-LB and Pirk mRNA from HKE-stimulated S2 cells transfected with dsRNA targeting GFP (negative control), Relish or Akirin (positive controls), and Hyd. (B) In vivo survival experiments performed on C564-Gal4/UAS-RNAi females Drosophila. They were infected with E. coli by septic injury (with PBS pricking as control). (C) Quantitative RT-PCR of Attacin-A and Attacin-D mRNA from C564-Gal4/UAS-RNAi Drosophila males infected with E. coli by septic injury. Data are represented as mean ± standard deviation of three independent experiments (A-C). After the ratio of stimulated over unstimulated values for each condition was determined, statistical significance was established by comparing genes knockdown with GFP dsRNA or RNAi control (B-C). *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001.