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. 2020 Apr 27;16(4):e1008458. doi: 10.1371/journal.ppat.1008458

Fig 4. Hyd/UBR5 is necessary for NF-κB target genes activation.

Fig 4

(A) Graphical representation of the predicted functional domains of Drosophila melanogaster Hyd and Homo sapiens UBR5. This representation highlights a conserved Ubiquitin-associated (UBA) domain, poly(A)-binding protein C-terminal (PABC) domain, Homologous to the E6-AP Carboxyl Terminus (HECT) domain and UBR type Zinc Finger (UBR ZF) domain. Annotation based InterPro software. Sequence similarity percentages calculated using the EMBOSS NEEDLE tool (default settings). (B) Quantitative RT-PCR of IL6, Ifit1, IL12β, IL8, Il1α and TNF mRNA from LPS-stimulated THP1 cells. They were transfected with scrambled siRNA (negative control) or siRNA targeting NF-κB1, AKIRIN2 (positive controls) or UBR5. Data are represented as mean ± standard deviation of three independent experiments. After the ratio of stimulated over unstimulated values for each condition was determined, statistical significance was established by comparing genes knockdown with scrambled siRNA control. *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001.