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M: protein marker; lane 1: lysates of recombinant bacteria incorporating pMAL-C2X/TsASP2 without induction; lane 2: lysates of recombinant bacteria incorporating pMAL-C2X/TsASP2 after induction; lane 3: lysate supernatant of recombinant bacteria incorporating pMAL-C2X/TsASP2 after induction; lane 4: sediment of recombinant bacteria incorporating pMAL-C2X/TsASP2 after induction; lane 5: purified rTsASP2. The arrow represents the band of rTsASP2 (86.4 kDa).
