Fig 2.
Western blot (A) and qPCR (B) analysis of TsASP2 protein and mRNA expression in different T. spiralis stages. A: Anti-rTsASP2 serum recognized native TsASP2 in different T. spiralis stage crude proteins, including ML (lane 1), 6-h IIL (lane 2), 3-d AW (lane 3), 6-d AW (lane 4), but not NBL (lane 5), and ML ES (lane 6) and IIL ES (lane 7). B: The TsASP2 mRNA expression levels in different T. spiralis stages were assessed by qPCR. Asterisks indicate a statistically significant difference compared with the ML stage (*P < 0.05).
