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. 2020 Apr 27;14(4):e0008269. doi: 10.1371/journal.pntd.0008269

Fig 2.

Fig 2

Western blot (A) and qPCR (B) analysis of TsASP2 protein and mRNA expression in different T. spiralis stages. A: Anti-rTsASP2 serum recognized native TsASP2 in different T. spiralis stage crude proteins, including ML (lane 1), 6-h IIL (lane 2), 3-d AW (lane 3), 6-d AW (lane 4), but not NBL (lane 5), and ML ES (lane 6) and IIL ES (lane 7). B: The TsASP2 mRNA expression levels in different T. spiralis stages were assessed by qPCR. Asterisks indicate a statistically significant difference compared with the ML stage (*P < 0.05).