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. 2020 Apr 23;5(8):e129905. doi: 10.1172/jci.insight.129905

Figure 7. JUN and JNK1 are sufficient to induce FOLFOX resistance and abrogate synergy between FOLFOX and JNK-IN-8.

Figure 7

(A) Representative images of CFPAC-1 cells transduced with pCW57-GFP-P2A-neo after 24 hours without or with 1 μg/mL dox. Scale bars: 100 μm. (B) Representative immunoblot showing dox induction of JUN transgene or control cells infected with empty vector (EV). Δ(Normalized growth +dox versus –dox) values for each line after FOLFOX (blue) or FOLFOX + JNK-IN-8 (purple), as calculated by subtracting growth after FOLFOX (2 μM 5-FU + 0.2 μM OX + 10 μM LEU) without dox from growth with FOLFOX with 1 μg/μL dox induction. Independent treatments and MTT assays were performed for FOLFOX versus FOLFOX + JNK-IN-8. Values shown are mean ± SEM (n = 3). (C) JNK1 transgene and (D) JNK2 transgene overexpression in similar experiments to B. KU80 used as loading control.