Figure 4. Chronic administration of AM-8123 increases EF and reduces collagen burden in an LAD ligation–induced MI rat model.

(A) Rats were treated with vehicle, AM-8123, losartan, or both. (B and C) Picrosirius red (PSR) staining of heart serial sections revealed less collagen deposition in the treated groups. (D) This was associated with a significant increase in EF. Data shown as individual animals (circles) and the group mean (bars) ± SEM. Data analyses were performed blinded. n = 9–10/group for histology. n = 9–14 for treated groups; n = 7 for sham for assessment of cardiac function. Significance was measured by a 1-way ANOVA with Tukey’s posttest (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).