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(A) Both AM-8123 and pyr-apelin-13 result in ERK phosphorylation in HUVECs grown under hypoxic conditions. Additionally, AM-8123 and pyr-apelin-13 exhibit a dose-dependent increase in both (B) ERK and (C) AKT phosphorylation in CHO cells overexpressing hAPJ. See full blots in Supplemental Figure 23. HTRF, homogeneous time resolved fluorescence.
