Figure 7. Acute administration of the APJ small-molecule agonist AM-8123 improves markers of cardiac function in beagles with tachypacing-induced HF under isoflurane anesthesia.

(A) Instrumented animals were subjected to an overdrive pacing protocol for approximately 4 weeks to induce HF. Pacemakers were turned off at least 15 minutes prior to the initiation of treatment. Vehicle (Veh) and AM-8123 were administered to anesthetized (Iso) animals in a randomized manner. For AM-8123, all canines were infused with escalating doses of AM-8123, resulting in increasing levels of (B) exposure and a dose-dependent increase in 2 load-insensitive measures: (C) PRSW and (D) ESPVR. Individual data points (circles) and group mean (bars) ± SEM are shown. Data analyses were performed blinded. Significance measured by a 2-way ANOVA with Tukey’s posttest (**P < 0.01; ***P < 0.001).