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. 2020 Apr 23;5(8):e131571. doi: 10.1172/jci.insight.131571

Figure 6. RNase 1 treatment of mice with polymicrobial sepsis resulted in a decreased cardiac apoptosis.

Figure 6

Heart tissue of sham-operated mice (n = 4) or mice with polymicrobial sepsis induced by CLP treated with vehicle or RNase 1 (both n = 5) were analyzed by Western blot. To analyze the intrinsic apoptosis signaling pathway, (A) Bax and (B) Bcl-2 levels as well as (C) caspase-9 and (D) caspase-3 activation were investigated. A 1-way ANOVA followed by Bonferroni test was used for multiple comparisons. Data are presented as dot plot with the mean ± SEM. Representative immunoblots are presented above the bar charts. The quantified OD of bands was corrected for the corresponding tubulin bands and normalized using the related sham band. Heart tissue of sham-operated mice treated with vehicle or RNase or mice with polymicrobial sepsis induced by CLP also treated with vehicle or RNase 1 (all n = 5) were analyzed by TUNEL labeling. (E) Apoptotic cells were labeled with TUNEL (red) and the nuclei of cardiomyocytes stained with DAPI (blue). Scale bars: 100 μm. (F) Quantification of TUNEL fluorescence. A 1-way ANOVA followed by Bonferroni test was used for multiple comparisons. Data are presented as dot plot with the mean ± SEM. §P < 0.05 versus Sham; #P < 0.05 versus CLP + Vehicle. CLP, cecal ligation and puncture; RNase, ribonuclease 1; Bcl-2, B-cell lymphoma 2.