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. 2020 Apr 23;5(8):e136907. doi: 10.1172/jci.insight.136907

Figure 3. Glp2r–/– mice exhibit dysglycemia and increased hepatic lipid deposition.

Figure 3

Metabolic endpoints in Glp2r+/+ and Glp2r–/– mice fed either a control diet (CD) or HFHC diet. (A) Seven- to eight-week-old Glp2r+/+ and Glp2r–/–male mice were allocated to CD or a HFHC diet for 17 weeks (n = 9–10/group for all panels). (B) Body weight gain. (C) i.p. glucose tolerance in 5-hour fasted mice and area under curve (AUC) 60–120 minutes. Data are presented as the mean ± SD. # P < 0.05, significant effect of genotype on AUC variance, using 2-way ANOVA with Tukey correction for multiple comparisons. (D and E) Plasma lipid profiles at endpoint. (F) Representative Oil Red O staining of liver sections. Scale bar: 100 μm. (G) Hepatic cholesterol and triglyceride (TG) content. (H) Hepatic mRNA abundance, relative to Ppia, of genes affecting lipid metabolism pathways. Graphs on the right illustrate relative mRNA levels of Hmgcr, Pparg, Cpt1a, and Hadhb genes, for which genotype had a significant effect on gene expression variability. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, §P < 0.05 significant effect of diet, #P < 0.05, significant effect of genotype on variance of gene expression, using 2-way ANOVA with Tukey correction for multiple comparisons.