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. 2020 Apr 23;5(8):e136907. doi: 10.1172/jci.insight.136907

Figure 8. Loss of GLP2R function does not effect CCl4-mediated liver injury and fibrosis.

Figure 8

Glp2r+/+ and Glp2r–/– mice were treated with a total of 4 CCl4 (0.7 ml/kg) i.p. injections (once every 3 days), and then sacrificed 3 days after the last CCl4 injection (n = 5/group). (A) Experimental design. (B) Plasma ALT levels in Glp2r+/+ mice compared with Glp2r–/– mice after completion of CCl4 course. (C) Representative Sirius red staining of liver sections (scale bar: 100 μm) and quantification of Sirius red–positive area. Relative mRNA levels of markers of fibrosis and stellate cell activation after completion of CCl4 administration in RNA from (D) whole liver normalized to Ppia, and (E) purified hepatic stellate cells (HSC) normalized to Pdgfrb. Data are presented as the mean ± SD. *P < 0.05, using Student’s t test.