Figure 2.

Multiphoton images from the proximal, mid and distal thoracic aorta (PTA, MTA and DTA) of 1 day old (do), 30do and 60do SR-BI^-/-/ApoeR61^h/h mice. Images depict 1-µm-thick optical slices captured from full-thickness z-stacks within the tunica media. Sections are representative of the thoracic aortic regions of interest, as shown in the in situ mouse preparation in Figure 1, with the head and tail ends of the mice at the top and bottom of each image, respectively. Collagen (green), elastin (red) and lipid (blue) signals are merged after post-image processing by Independent Component Analysis (ICA). Scale, 50 µm.