Figure 4.
Three-dimensional, multiphoton, maximum projection renderings (approximate size 512×512×150-200 µm) from z-stacks of the proximal ventral (anterior) thoracic aortas (VTAs) of SR-BI-/-/ApoeR61h/h mice aged 1, 30 and 60 days old (do). Collagen second harmonic generation (SHG) is green, elastin auto-fluorescence is red and Nile Red (NR) lipid droplet fluorescence is blue. Independent component analysis (ICA) was applied to all z-stacks for post-image processing. For a more detailed view, please refer to the movies in the Supplemental Materials. Scale bar, 50 µm.