Fig3 video1.
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DOI: 10.1369/0022155414559590.M1
AVI video clips were produced from a full-thickness z-stack (ranging from ~150–200-µm-thick tissue image acquisitions) obtained within the ventral (anterior) surface of the proximal thoracic aorta of a representative SR-BI-/-/ApoeR61h/h mouse aged 60 days old (do). En-face projections coupled with cross sections are provided. Collagen second harmonic generation (SHG) is green, elastin auto-fluorescence is red and Nile Red (NR) lipid droplet fluorescence is blue. Image acquisition was optimized during all experiments to fill the digitizer. ICA was applied during post-image processing and the elastin fluorescence signal was optimized across all age groups for comparison purposes. Additional movies are provided in the Supplemental Materials.