Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | DH5α(λpir) | Gibco BRL | F– hsdR17 deoR recA1 endA1 phoA supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 ϕ80dlacZΔM15 ****add pir | |
| Strain, strain background (Escherichia coli) | TB28 | (Bernhardt and de Boer, 2003) | MG1655 ΔlacIZYA::frt | |
| Strain, strain background (Corynebacterium glutamicum) | MB001 | (Baumgart et al., 2013) | ATCC 13032 ΔCGP1 (cg1507-cg1524) ΔCGP2 (cg1746-cg1752) ΔCGP3 (cg1890-cg2071) |
|
| Strain, strain background (Corynebacterium glutamicum) | HL18 | This work | MB001 ΔponA | Bernhardt lab (MB001/pHCL86, see Materials and methods) |
| Strain, strain background (Corynebacterium glutamicum) | JS5 | This work | MB001 Δcgp_3012-cgp_3020 | Bernhardt lab (MB001/pJWS1, see Materials and methods) |
| Strain, strain background (Corynebacterium glutamicum) | JS6 | This work | MB001 Δcgp_3019 | Bernhardt lab (MB001/pJWS2, see Materials and methods) |
| Strain, strain background (Corynebacterium glutamicum) | JS7 | This work | MB001 Δcgp_3018 | Bernhardt lab (MB001/pJWS3, see Materials and methods) |
| Strain, strain background (Corynebacterium glutamicum) | JS8 | This work | MB001 Δcgp_0016 | Bernhardt lab (MB001/pJWS4, see Materials and methods) |
| Strain, strain background (Corynebacterium glutamicum) | JS10 | This work | MB001 Δcgp_0016 ΔponA | Bernhardt lab (JS8/pHCL86, see Materials and methods) |
| Recombinant DNA reagent | pJWS1 | This work | KanR, pCRD206 derivative containing an insert covering upstream and downstream of cgp_3012-cgp_3020. | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS2 | This work | KanR, pCRD206 derivative containing an insert covering upstream and downstream of cgp_3019. | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS3 | This work | KanR, pCRD206 derivative containing an insert covering upstream and downstream of cgp_3018. | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS4 | This work | KanR, pCRD206 derivative containing an insert covering upstream and downstream of cgp_0016 (cofA). | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS18 | This work | KanR, pK-PIM derivative encoding Psod-riboE1-mscar-cgp_0016 (cofA) | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS19 | This work | KanR, pK-PIM derivative encoding Psod-riboE1-mscar-cgp_0336 (ponA) | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS97 | This work | KanR, pK-PIM derivative encoding Psod-riboE1-mscar-C. gluponA (E. coliTM) | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS102 | This work | KanR, pK-PIM derivative encoding Psod-riboE1-mscar-cgp_0336 (ponA) GT- (E97A) | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS103 | This work | KanR, pK-PIM derivative encoding Psod-riboE1-mscar-cgp_0336 (ponA) TP- (S393A) | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS104 | This work | KanR, pK-PIM derivative encoding Psod-riboE1-mscar-cgp_0336 (ponA) GT- and TP- (E97A and S393A) | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pHCL86 | (Lim et al., 2019) | KanR, pCRD206 derivative containing an insert covering upstream and downstream of cgp_0336 (ponA). | |
| Recombinant DNA reagent | pHCL149 | (Lim and Bernhardt, 2019) | CmR, Para-popZ-rbs-H3H4-msfGFPN-tmponB. | |
| Recombinant DNA reagent | pHCL152 | (Lim and Bernhardt, 2019) | TetR, lacI-Plac-mscar. | |
| Recombinant DNA reagent | pJWS29 | This work | CmR, Para-popZ-rbs-H3H4-msfGFP-ponA | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS41 | This work | TetR, lacI-Plac-mscar-cgp_0016 | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS70 | This work | TetR, lacI-Plac-mscar-jk0012 | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS73 | This work | CmR Para-popZ-rbs-H3H4-msfGFP-ponATM | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS75 | This work | CmR, Para-popZ-rbs-H3H4-msfGFP-ponASwap | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS78 | This work | CmR, Para-popZ-rbs-H3H4-jk1977TM | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS80 | This work | CmR, Para-popZ-rbs-H3H4-msfGFP-ponB | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS81 | This work | CmR, Para-popZ-rbs-H3H4-ponA2TM | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS83 | This work | TetR, lacI-Plac-mscar-rv0007FL | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS88 | This work | CmR, Para-popZ-rbs-H3H4-ponA1TM | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS90 | This work | CmR, Para-popZ-rbs-H3H4-jk2069TM | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS114 | This work | TetR, lacI-Plac-mscar-rv0007ΔN | Bernhardt lab (see Supplementary file 2) |
| Recombinant DNA reagent | pJWS119 | This work | CmR, Para-popZ-rbs-H3H4-C. gluponA (E. coliTM) | Bernhardt lab (see Supplementary file 2) |
| Chemical compound | Bocillin | ThermoFisher Scientific | BOCILLIN FL Penicillin, Sodium Salt |