Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Monoclonal α-His | Sigma-Aldrich | SAB1305538 | Mouse origin; 1:2000 dilution |
| Antibody | Polyclonal α-mouse-HRP conjugate | Sigma-Aldrich | AP130P | Goat origin; 1:20,000 dilution |
| Chemical compound, drug | β-n-dodecyl β-D-maltoside (DDM) | Carl Roth | CN26.5 | |
| Chemical compound, drug | Bovine brain lipid extract | Sigma-Aldrich | B1502 | |
| Chemical compound, drug | E. coli polar lipids | Avanti | 100600 | |
| Chemical compound, drug | 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine (DOPC) | Anatrace | D518 | |
| Chemical compound, drug | [2,5’,8-3H(N)]-ATP (3H-ATP) | PerkinElmer | NET118900 | |
| Chemical compound, drug | [2,8-3H]-ADP (3H-ADP) |
Biotrend | ART-0386 | |
| Chemical compound, drug | [α32P]-ATP | Hartmann Analytic | FP-207 | |
| Chemical compound, drug | [γ32P]-ATP | Hartmann Analytic | FP-201 | |
| Chemical compound, drug | Copper-chelated PVT SPA beads | PerkinElmer | RPNQ0095 | |
| Gene (Thermus thermophilus) | TmrA | Q72J05 | TTC0976 | |
| Gene (Thermus thermophilus) | TmrB | Q72J04 | TTC0977 | |
| Peptide, recombinant protein | RRY-[3H]L-KSTEL | Hartmann Analytic | Custom synthesis | |
| Peptide, recombinant protein | RRY-C*-KSTEL | This study (Peptide labeling and purification) | C* denotes fluorescein labeled Cys | |
| Peptide, recombinant protein | RRY-C*-KSTEL | This study (Peptide labeling and purification) | C* denotes Alexa Fluor 647 labeled Cys | |
| Recombinant DNA reagent | pET-22b | Merck Millipore | 69744 | Vector for protein expression in E. coli |
| Software, Algorithm | Prism 5 | GraphPad | ||
| Software, Algorithm | PyMOL | Schrödinger | ||
| Strain, strain background (Escherichia coli) | BL21(DE3) | Thermo Fisher | C600003 | Chemically competent cells |