FIG 1.

Influence of different iron concentrations on growth, pH profile, and by-product formation of C. glutamicum wild type during cultivation in CGXII minimal medium with 222 mM glucose and either 36 μM FeSO₄ (black symbols) or 1 μM FeSO₄ (red symbols). (A) Growth was measured as the optical density at 600 nm (OD₆₀₀). (B to H) Aliquots of the culture supernatants were analyzed with respect to pH (B), glucose consumption (C), and formation of pyruvate (D), acetate (E), l-alanine (F), 2-oxoglutarate (G), and lactate (H). Mean values and standard deviations of three biological replicates are shown. The data for the C. glutamicum wild type cultivated with 36 μM FeSO₄ were taken from a previous study (26).