FIG 2.
Influence of different iron concentrations and thiamine on growth, pH profile, and by-product formation of C. glutamicum wild type during cultivation in CGXII minimal medium with 111 mM glucose and either 36 μM FeSO4 (black symbols), 1 μM FeSO4 (red symbols), or 1 μM FeSO4 plus 0.6 μM thiamine (blue symbols). (A) Growth was measured as the OD600. (B to H) Aliquots of the culture supernatants were analyzed with respect to pH (B), glucose consumption (C), and formation of pyruvate (D), acetate (E), l-alanine (F), 2-oxoglutarate (G), and lactate (H). Mean values and standard deviations of the results from three biological replicates are shown.