TABLE 3.
TnFLXPhyi mutants
| Strain | Phenotypea | IPTGb | Insertion | Tagc | Annotation |
|---|---|---|---|---|---|
| DK7507 | Blue | Yes | yesX::TnFLXPhyi cat | TATCATCGG | Induces yesZ |
| DK7508 | Blue | Yes | yesS::TnFLXPhyi cat | TATGGCCGC | Induces yesZ |
| DK7509 | Blue | Yes | yesS::TnFLXPhyi cat | TATGGAGAG | Induces yesZ |
| DK7510 | Blue | Yes | ganQ::TnFLXPhyi spec | TAACCTTTA | Induces ganA |
| DK7525 | Blue | Yes | yesW::TnFLXPhyi spec | TAGTTGCCT | Induces yesZ |
| DK7527 | Blue | Yes | yesX::TnFLXPhyi spec | TATGGTGTA | Induces yesZ |
| DK7505 | Blue | No | ganR::TnFLXPhyi tet | TATGTGGGC | Derepresses ganA |
| DK7692 | Blue | Yes | yesQ::TnFLXPhyi tet | TAATGCTCG | Induces yesZ |
| DK7506 | Blue | Yes | yesR::TnFLXPhyi tet | TACAAAATA | Induces yesZ |
| DK7528 | Blue | Yes | yesQ::TnFLXPhyi kan | TATATTATG | Induces yesZ |
| DK7530 | Blue | No | ganR::TnFLXPhyi kan | TAAGTCATC | Derepresses ganA |
| DK7532 | Blue | Yes | yesT::TnFLXPhyi kan | TACCTTGTT | induces yesZ |
a
Blue/white phenotype on plates containing X-Gal.
b
Whether IPTG is required in the medium to obtain a blue colony phenotype on X-Gal.
c
The tag is the first 9 bases immediately adjacent to the transposon inverted terminal repeat and is included to allow precise location of the insertion.