TABLE 5.
Fluorescent TnFLXgfp mutants
| Strain | Localization | Insertion | Taga | Annotation |
|---|---|---|---|---|
| DK7554 | Membrane | mntB::TnFLXgfp cat | TAAGGCTGA | Manganese transporter |
| DK7657 | Diffuse | iolG::TnFLXgfp cat | TATCAATCT | Inositol dehydrogenase |
| DK7658 | Diffuse/punctate | clpC::TnFLXgfp cat | TAGAAGATG | Protease subunit |
| DK7755 | Diffuse | dps::TnFLXgfp kan | TAGGTTAAG | Iron storage protein |
| DK7552 | Punctate | cheV::TnFLXgfp kan | TAGAGGACT | Chemotaxis protein |
| DK7662 | Membrane | fliG::TnFLXgfp kan | TATCTTCTG | Flagellar rotor protein |
| DK7547 | Diffuse | fadR::TnFLXgfp tet | TATCCAAAA | Transcriptional repressor |
| DK7548 | Punctate | cheV::TnFLXgfp tet | TATCAAAAC | Chemotaxis protein |
| DK7549 | Membrane | flhA::TnFLXgfp tet | TAGATCCGA | Flagellar secretion protein |
| DK7560 | Diffuse | spoVG::TnFLXgfp spec | TAGATTCGA | RNA-binding protein |
| DK7559 | Filament | spoVN::TnFLXgfp spec | TAGGCTATG | Alanine dehydrogenase |
| DK7558 | Membrane | floT::TnFLXgfp spec | TAATTGCAC | Membrane scaffold protein |
a
The tag is the first 9 bases immediately adjacent to the transposon inverted terminal repeat and is included to allow precise location of the insertion.