Figure 2. hRpn2-bound hRpn13 interacts dynamically with ubiquitins of K48-diubiquitin.

(A) Expanded regions for ubiquitin A46 from a ¹³C-edited NOESY spectrum (100 ms mixing time) on sample Ternary-¹³C-P (Figure 1A, left panel) or Ternary-¹³C-D (Figure 1A, right panel). NOEs between A46 signals from the free and bound states are labeled and indicated with dashed black lines whereas intermolecular NOEs to hRpn13 are labeled and indicated with a solid purple line. (B) Schematic representation of exchangeable states presence in sample Ternary-¹³C-P or Ternary-¹³C-D with coloring as in Figure 1A.