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. 2020 May 7;3:216. doi: 10.1038/s42003-020-0941-1

Fig. 4. Phos-tag SDS-PAGE and LC-MS/MS and analyses confirmed the phosphorylation at residue S78.

Fig. 4

a LC-MS/MS analysis of the upper band of wildtype TglT sample by Phos-tag SDS-PAGE, confirming that it was phosphorylated at S78. See the detailed results in Supplementary Fig.  5. b LC-MS/MS analysis of the band in sample S78A mutant co-expressed with TakA by Phos-tag SDS-PAGE, confirming that the S78A mutation prevented the phosphorylation. See the detailed results in Supplementary Fig.  6. c TglT and its variants expressed in the presence and absence of TakA were analyzed by Phos-tag SDS-PAGE providing maximal separation between the phosphorylated and non-phosphorylated species. The mutations from the five conserved motifs are annotated; conserved motifs are color-coded as in Fig. 3. Source data are provided as a Supplementary Data 2.