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. 2020 May 7;3:216. doi: 10.1038/s42003-020-0941-1

Fig. 7. TakA (Rv1044) autoregulates the promoter of Rv1044-Rv1045 TA.

Fig. 7

a Schematic diagram of the PRv1044-lacZ fusion reporter in M. smegmatis. A PRv1044-lacZ reporter containing a 460 bp homology sequence of MSMEG_1282 gene, a T4_32 terminator (32 bp), a 500 bp upstream of Rv1044 gene and a 501 bp homology sequence of lacZ gene was inserted into the chromosome of M. smegmatis. b The promoter activity of Rv1044 was assessed with empty vector (vector), the plasmid expressing Rv1044 (the antitoxin) or the plasmid expressing Rv1044 and Rv1045 (antitoxin-toxin). The experiments were conducted in the absence and presence of anhydrotetracycline (ATC), respectively. Data are presented as a scatterplot showing the mean and standard error of three independent experiments (each dot represents a biological replicate). Asterisks represent statistically significant differences using the one way ANOVA analyses. ***p < 0.0001. Source data are provided as a Supplementary Data 2.