Fig. 6.

CaM inhibition by calmidazolium rescued erianin-induced ferroptosis. a The effect of blocking Ca²⁺/CaM signaling with calmidazolium on erianin-induced inhibition of growth in H460 cells, **p < 0.01. b The effect of blocking Ca²⁺/CaM signaling with calmidazolium on erianin-induced inhibition of growth in H1299 cells, **p < 0.01. c Intracellular chelatable iron in H460 and H1299 cells after treatment with a combination of calmidazolium and erianin was determined using the fluorescent indicator Phen Green SK (green). d The intracellular Ca²⁺ concentration in H460 and H1299 cells after treatment with a combination of calmidazolium and erianin was detected by measuring the fluorescence intensity of the calcium indicator Fluo-3/AM with fluorescence microscopy. e Western blotting showed the effect of blocking Ca²⁺/CaM signaling with calmidazolium treatment for 24 h on erianin-induced ferroptosis in lung cancer