Fig. 5. Effect of medium on growth of Synechococcus sp. PCC 11901.

Analysis of the effect of medium supplementation with increasing concentrations of sodium nitrate, iron (III) chloride and potassium dihydrogen phosphate on growth of PCC 11901. Growth conditions: 38 °C, 200 rpm, 300 µmol photons m⁻² s⁻¹ light intensity (RGB LED 4:2:1 ratio) and 1% CO₂. a AD7 medium with double the regular concentration of FeCl₃ (30 µM) was supplemented with a wide spectrum of NaNO₃ concentrations. b AD7 medium enriched with 96 mM NaNO₃ was supplemented with different concentrations of FeCl₃. c, d Modified AD7 medium with 96 mM NaNO₃ and 240 µM FeCl₃ was supplemented with increasing concentrations of KH₂PO₄ and tested for growth and biomass accumulation over 10 days. Data points with error bars represent mean of n = 3 biological replicates ± standard deviation.