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. 2020 May 7;3:217. doi: 10.1038/s42003-020-0954-9

Fig. 4. Effects of single-site mutations on methanol-dependent growth and methanol tolerance.

Fig. 4

a Effects of single-site mutations on methanol-dependent growth of strain MX-10. Strain MX-10 and its derivatives harboring single-site mutations were cultivated using LB medium supplemented with 4 g/L methanol and 4 g/L xylose as the carbon sources. OD600nm values after 72 h cultivation were shown. *P < 0.05, ***P < 0.001, one-way ANOVA, N = 3; cgl0653G1256A, P = 0.033; cgl0754C582A, P = 0.17; cgl0833C1439T, P = 5.1 × 10−6; cgl1367C584T, P = 8.1×10−5; cgl1520A574G, P = 2.5 × 10−4; cgl2365C542G, P = 1.3 × 10−6; cgl2857G183A, P=2.8 × 10−5; cgl2998G104T, P = 0.11. b–d Growth curve of C. glutamicum ATCC 13032 wild-type strain and its derivatives harboring single-site mutations in CGXII minimal medium supplemented with 5 g/L glucose (b), 5 g/L glucose and 30 g/L methanol (c), and 5 g/L glucose and 15 mg/L formaldehyde (d). ***P < 0.001, one-way ANOVA, N = 3; cgl0653G1256A vs. wild-type, P = 2.4 × 10−5; cgl0833C1439T vs. wild-type, P = 1.9 × 10−6; cgl2857G183A vs. wild-type, P = 3.8 × 10−5; cgl2998G104T vs. wild-type, P = 4.0 × 10−4. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).