Fig. 8. Single-cell expression clustering of T and B cell signaling genes.

a RNA expression of T cell and B cell signaling protein-coding genes demonstrated via viSNE plots of a 50:50 mixture of Jurkat and Nalm6 cell lines. Data from an InsRT-QBC experiment was rendered via viSNE using 14 of the 16 targets as selected channels to organize the viSNE plots. The housekeeping markers GAPDH, ACTB were deliberately excluded from the viSNE run to view how the expression of the other markers correlated with each other. Anti-expression of the T and B cell markers is clearly visible. Plots represent 51,760 cells. Two additional biological replicates are in Supplementary Fig. 11. b Bar graph of the percentage of cells expressing each RNA target from n = 3 biologically independent cell samples. Single-cell bivariate plots were hand gated for cells positive for the RNA targets. The average percentages for the three replicates were plotted as bars and individual data points for the three replicates were overlaid. RNA targets only expected to be expressed in T cells (red) or B cells (green) have percentages at or below ~50% while all but one RNA target expected to be expressed in both cell types (blue) are detected in >50% of the measured single cells. The housekeeping markers, GAPDH and ACTB, were measured in ~99% of cells.