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. 2020 May 7;3:218. doi: 10.1038/s42003-020-0938-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Batch registration of the SDY420 study was performed by registering each of the internal standards to a reference (Batch 4) internal standard to generate 24 batch-specific registration templates, and the shifts in these templates were applied to the appropriate batches of individual samples (total 260 samples). For three batches, consensus reference samples were used because internal standards were unavailable. A concatenate of the registered samples was clustered in SWIFT, and all samples were assigned to the resulting cluster template. QC plots (described in Fig. 1) show the variation in the centroids of 13 selected channels of each cluster in all internal standards before and after registration (a) and in 12 actual samples from one batch before and after batch registration (b). Arrows indicate samples that are outliers for CD20 or CD45RA cluster centroids.