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. 2020 May 7;3:218. doi: 10.1038/s42003-020-0938-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — The registered and non-registered samples described in Fig. 7 were analyzed manually using a single set of gates without any algorithmic assistance. The same gates (Supplementary Fig. 6b) were used to analyze both registered and non-registered data. Each dot represents one subject. Subpopulation sizes are sorted by age or batch for naive CD4 T cells or naive CD8 T cells. p Values were derived from a two-sided Wilcoxon test of two subject groups (oldest vs. youngest) with n = 20 biologically independent samples per group.