Fig. 7.

Two pore channel inhibition prevents SV40 disassembly and exposure of minor capsid proteins.

Vero cells were chilled at 4 °C for 1 h before addition of SV40 virions at an MOI of 3 in chilled growth medium. Cells were maintained at 4 °C for 1 h with occasional agitation to facilitate binding. Pre-warmed growth medium containing drug was then added prior to incubation at 37 °C for 10 h before fixation. Following permeabilisation, immunostaining was performed to detect SV40 VP2/3 and the ER using calnexin. DAPI was used to visualise nucleic acids.