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. 2020 Apr 21;23(5):101083. doi: 10.1016/j.isci.2020.101083

Figure 2.

Figure 2

Characterization of Human iPNECs

(A) Representative immunofluorescence (IF) images of ALI cultures at Day 31 of directed differentiation: SYP+ iPNECs (red), NKX2.1, EPCAM, and vimentin (VIM) (all green). Left panels (20×) represent low-power images (scale bars, 200 μm), and right panels (40×) show magnified views of the marked areas (scale bars, 10 μm).

(B) Representative IF images of adult human distal lung sections: SYP+ iPNECs (green), NKX2.1, EPCAM, and VIM (all red). Yellow dotted lines demarcate the airways. Left panels (20X) represent low-power images, and right panels (40×) show magnified views of the marked areas (scale bars, 50 μm). Nuclei are counterstained with DAPI (blue).