FIGURE 5.

Wnt signalling pathway is inhibited in WTAP‐deficient endothelial cells. A, qRT‐PCR analysis of the mRNA levels of specific genes in WTAP‐deficient or (B) overexpressing endothelial cells. C, Western blot analysis of the protein level of β‐catenin after silencing or overexpressing WTAP. D, Immunofluorescent staining for β‐catenin in WTAP‐deficient or (E) overexpressing endothelial cells. Data are shown as mean ± SEM of three independent experiments. P values were calculated using Student's t test. *P < .05; **P < .01; ***P < .001