Figure 2.

PTBP1 deficiency enhances cisplatin efficacy in CISR osteosarcoma cells. A, The effect of PTBP1 knock‐down on cell viability of CISR osteosarcoma cells was measured by CCK‐8 assay. B, The effect of PTBP1 knock‐down on cisplatin‐induced inhibition of cell viability of CISR osteosarcoma cells was verified by CCK‐8 assay. C, Colony formation assay examined the effects of PTBP1 knock‐down, cisplatin alone and PTBP1 knock‐down combined with cisplatin on colony formation ability of CISR osteosarcoma cells. D, Flow cytometric analysis was used to detect the effects of PTBP1 knock‐down, cisplatin alone and PTBP1 knock‐down combined with cisplatin on cell apoptosis of CISR osteosarcoma cells. E, The effect of sh‐PTBP1 on cell viability of MG‐63 and U‐2OS cells was measured by CCK‐8 assay. F, The effect of sh‐PTBP1 on cisplatin‐induced inhibition of cell viability of MG‐63 and U‐2OS cells was verified by CCK‐8 assay. *P < .05, **P < .01, n.s is no significance