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. 2020 Mar 24;24(9):5274–5289. doi: 10.1111/jcmm.15183

Figure 4.

Figure 4

SLC31A1 is down‐regulated in chemotherapy‐insensitive osteosarcoma tissues and CISR osteosarcoma cells. A, The level of SLC31A1 mRNA in osteosarcoma tissues was measured by qRT‐PCR. B, Linear regression analysis of the correlation between the expression levels of SLC31A1 and PTBP1 in osteosarcoma. C, SLC31A1 expression in GR tissues and PR tissues was measured by IHC. Scale bars: 500 μm (25×) and 50 μm (200×). D and G, The expression of SLC31A1 in MG‐63, U‐2OS and their cisplatin‐resistant sub‐lines was measured by qRT‐PCR and Western blot. E and H, The expression of SLC31A1 in MG‐63CISR, U‐2OSCISR cells and PTBP1 knock‐down MG‐63CISR, U‐2OSCISR cells was measured by qRT‐PCR and Western blot. F and I, The expression of SLC31A1 in MG‐63, U‐2OS cells and PTBP1 knock‐down MG‐63, U‐2OS cells was measured by qRT‐PCR and Western blot. *P < .05, **P < .01