Figure 2.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1. A, Overexpression of HDAC1 was assessed by Western blotting. The relative protein levels were normalized to GAPDH. B, NRCF proliferation was assessed by RTCA every 15 min. Electrical impedance was normalized according to the background measurement at time point 0. C and D, The expression of collagen I, t‐Akt, p‐Akt, p‐Smad2/3, p53 and Ac‐p53 was assessed by Western blotting. The relative protein levels were normalized to GAPDH; protein levels of p‐Akt were normalized to t‐Akt. E and F, Nuclear protein levels of HDAC1, AcH3, AcH3K18 and AcH3K27 were assessed by Western blotting. Relative protein levels were normalized to histone 3 (H3). G, ChIP analysis of the recruitments of acetylated H3K18 and H3K27 into p21 promoter in NRCFs. Data are shown as the means ± standard errors of the mean of triplicates and are representative of three independent experiments performed. *P < .05, **P < .01, ***P < .001, ****P < .0001