FIGURE 2.

The effect of prostaglandin analogues or beta‐blockers alone and in combination on the differential gene expression of fibrosis‐associated genes in human trabecular meshwork cells in vitro. Graphs indicate mean mRNA expression of TGFβ1, TGFβ2, TGFβR2, CTGF, FN, LOXL2, WNT3A, DECORIN and HEVIN normalized to expression of β‐ACTIN (housekeeping gene) in human TM cells in vitro following IOP‐lowering medication for 24 h. The categories include untreated controls (Ctrl), prostaglandin analogue (PG), beta‐blocker (BB) or combination of prostaglandin analogue and beta‐blocker (PG+BB) treatments. Bar graphs represent the mean ± SEM of six independent experiments. *P < .05, **P < .01, ***P < .001, Mann‐Whitney test