Figure 5.

Effect of SLA class I silencing effect on NK cell xenogeneic immune response. ICCs‐derived cells were co‐culture with NK cells for 4 h, and degranulation was measured by flow cytometry. A, Levels of CD107a expressed in NK cells cultivated alone (NK Ctrl), control NT cells (Ctrl) and from genetically engineered ICCs‐derived cells (shNS and shβ2M). B, Representative histogram plots from spontaneous NK cell degranulation, C, NK cell degranulation towards control NT ICCs‐derived cells, D, CD107a expression after NK cell coculture with shNS cells and E, NK cell co‐culture with SLA class I‐silenced ICCs‐derived cells. Statistical significance was calculated using one‐way ANOVA followed by Tukey's post hoc test. **P < .01; ***P < .001