Figure 6.

SLA silencing prevents antibody‐mediated cellular‐dependent cytotoxic responses. The strength of humoural immune response was assessed by performing antibody‐mediated cell‐dependent cytotoxicity (ADCC) assays. The activation of Jurkat effector cells was measured by detecting bioluminescence activity after 6 h incubation with ICCs‐derived cells with and without anti‐SLA‐specific antibodies. Graph shows relative luminescence units (RLU) detected in ADCC assays after effector cell exposure to ICCs‐derived cells in the presence or absence of A, anti‐SLA class I or B, anti‐SLA class II‐specific antibodies. Graphs show means and standard deviations of four independent ADCC assays. Statistical significance was calculated using one‐way ANOVA followed by Tukey's post hoc test. *P < .05; ***P < .001