FIGURE 2.

PiR‐63076 mediated hypoxia‐induced PASMC proliferation but not piR‐62974. A, The expression of PCNA was increased in hypoxia which was magnified by piR‐63076 agomir and reversed by piR‐63076 antagomir. B, The protein expression of cyclin A, cyclin D and cyclin E in PASMCs under different conditions. C, Fluorescent EdU incorporation (red) co‐stained with Hoechst (blue to demonstrate nuclear) proved that hypoxia‐induced DNA synthesis was blocked by piR‐63076 inhibitor. Scale bar = 50 μm. D, Fluorescence‐activated cell sorting analyses detected the number of cells in each phase of the cell cycle in PASMCs. *P < .05, **P < .01 compared with normoxia. ^# P < .05, ^## P < .01 compared with hypoxia and NC. n = 6. All the values are denoted as mean ± SEM