FIGURE 3.

In situ hybridization and RNA‐fluorescent in situ hybridization (FISH) assay showed the distribution of piR‐63076 under hypoxic conditions. A, Localization and quantitative analysis of positive staining per vascular area of piR‐63076 and NC expression in lung tissue. B, FISH assay was performed to detect piR‐63076 expression in cultured cells. U6 and 18S RNA were used as controls for localization of the nucleus and cytoplasm. Scale bar = 50 μm. *P < .05. n = 4. All values are denoted as mean ± SEM