FIGURE 5.

The association between piRNA‐63076 and methylation. A, The protein expression levels of Dnmt1, Dnmt 3a and Dnmt 3b. B, Global DNA methylation changes induced by piR‐63076 in PASMCs was measured after hypoxia and transfection. C and D, Demethylation by decitabine and azacitidine increases the expression of Acadm in hypoxia, but not Stat1 and Maoa. E, Schematic of the CpG islands within the Acadm promoter. Nor, normoxia; Hyp, hypoxia. *P < .05, **P < .01 compared with normoxia. ^# P < .05, ^## P < .01 compared with hypoxia plus NC. n = 6. All of the values are denoted as mean ± SEM