FIGURE 7.

Effect of Acadm on PASMC proliferation in hypoxia. A, The expression of Acadm in vivo and in vitro was detected by Western blot. B, Protein expression of intracellular Acadm was measured to assess the efficiency and specificity of plasmid targeting Acadm overexpression. C, The protein expression of PCNA was determined by Western blot analysis. D, Acadm protein overexpression was measured by Western blot in hypoxia. E, Proliferation of PASMCs was evaluated by EdU incorporation assay. Scale bar = 50 μm. F, The protein expression levels of cyclin A, cyclin D and cyclin E. G, The number of cells in each phase of the cell cycle was examined by flow cytometry in normoxia or hypoxia. Nor, normoxia; H and Hyp, hypoxia; ACA, Acadm overexpression. *P < .05, **P < .01 compared with normoxia. ^# P < .05, ^## P < .01 compared with hypoxia plus NC. n = 6. All of the values are denoted as mean ± SEM