FIGURE 3.
Blockage of FABP2 suppresses HFHC diet–induced intestinal permeability in ApoE−/− mice. Male ApoE−/− mice were fed with HFHC for 12 wk, then treated with 1 × 109 lentiviral particles encoding villin‐drived Fabp2 siRNA or control siRNA (Veh) for 8 wk. A, Haematoxylin and eosin staining of small intestine. Scale bar = 100 μm. B‐C, Real‐time PCR analysis of intestinal zonula occludens (ZO)‐1 (B) and occludin (C) levels. D‐F, Western blot analysis of ZO‐1 and occluding (D), and quantitative analysis of relative density of ZO‐1/Tubulin (E) and occluding/Tubulin (F). G, Serum levels of lipopolysaccharides (LPS). H, The circulating concentration of DX‐4000‐FITC after mice was orally administrated with FITC‐labelled dextran. Data are shown as mean ± SEM (*P < .05, **P < .01 and ***P < .001, Student's t test was used for 2‐group comparison, n = 6‐7 mice/group)