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. 2020 Mar 27;24(9):5363–5374. doi: 10.1111/jcmm.15192

Figure 5.

Figure 5

Six1 reintroduction reverses the promotional effect of miR‐155‐3p. A, Relative expression of miR‐155‐3p in A172 and U87 cells was analysed by real‐time PCR. B, Cell viability was analysed in A172 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. C, Cell viability was analysed in U87 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. D, Edu assay in A172 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. E, Colony formation was analysed in A172 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. F, Colony formation was analysed in U87 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. G, Cell cycle was analysed by flow cytometry in A172 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. H, Cell cycle was analysed by flow cytometry in U87 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. I, Western blotting of indicated protein in A172 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. J, Western blotting of indicated protein in U87 cells transfected with miR‐con or miR‐155‐3p with or without Six1 overexpression. Results were expressed as means ± SD of 3 independent experiments. *P < .05; **P < .01