Figure 4.

SAAG, SA and AG attenuated ASK1 activation and subsequent activation of the JNK and p38 signalling cascades. A, Western blotting of ASK1, p‐ASK1 and the quantitation of p‐ASK1/ASK1 (n = 3); B, Immunofluorescence staining for p‐ASK1 (green) and NeuN (red) and the quantitation of fluorescence intensity of p‐ASK1 (arbitrary units, n = 3‐5 animals per group); scale bar: 50 μm. C, Western blotting of JNK, p‐JNK, p38, p‐p38, p‐c‐Jun, c‐Jun, MAPKAPK2 and p‐MAPKAPK2 levels and their quantitation (n = 3). ^# P < .05 compared with the sham group, *P < .05 compared with the I/R group, and ^∆ P < .05 compared with the I/R + SAAG group