Figure 6.

Inhibition of the Trx system reversed the protective effect of SAAG. A, Images of TTC staining, infarction rate and neurological deficient scores (n = 6). B, SOD activity and MDA content (n = 6). C, Caspase‐3 activity (n = 6), D, GSH levels (n = 6); E, Western blotting of Nrf2 and its quantitation (n = 3). F, Immunofluorescence staining for cleaved caspase‐3 (red), 8‐OHdG (green) and their quantitation of fluorescence intensity (arbitrary units, n = 3‐5 animals per group); nuclei (blue), scale bar: 50 μm. G, Immunofluorescence staining for Nrf2 (red), NQO1 (green) and their quantitation (arbitrary units, n = 3‐5 animals per group); nuclei (blue), scale bar: 50 μm. ^# P < .05 compared with the sham group, *P < .05 compared with the I/R group, and ^∆ P < .05 compared with the I/R + SAAG group