Figure 5.

Transcription factor FOXO3 promotes ABCB1 transcriptional level. A, Western blot indicated the ABCB1 protein in the HCC cells after the overexpression of transcription factor FOXO3. B, Bioinformatics analysis indicated the binding sites of FOXO3 on the ABCB1 gene promoter. C, Luciferase reporter vectors for the assay were constructed, including wild‐type and the site‐mutant controls. D, Luciferase reporter assay indicated the activity of the cotransfection of FOXO3 with first site (GTAAACA) and second site (GTAAATT). E, Chromatin immunoprecipitation (ChIP) followed by PCR analysis indicated the enrichment in the FOXO3 antibody or control. F, Public database (GEPIA, http://gepia.cancer-pku.cn/) illustrated the positive correlation with FOXO3 and ABCB1 expression. Data are the means ± SD. **P < .01