Figure 4.

The up‐regulation of Sirt3 expression by HKL improves FAO dysfunction and renal function. A, Western blot (n = 4) and real‐time PCR analysis (n = 3) of Sirt3 in the renal tissue of control and cisplatin‐treated mice after saline or HKL administration, **P < .01. B, Comparison of renal function evaluated as serum creatinine and blood urea nitrogen in mice in each group, ***P < .001, **P < .01, n = 6. C, Comparison of renal histological changes evaluated as number of cast and necrotic tubules per HPF. ***P < .001, **P < .01. D, Oil red O staining showed lipid deposition in the kidneys of mice in each group, scale bar, 200 µm. E, Comparison of the FFA concentrations in kidneys from mice in each group, ***P < .001, n = 6. F, Western blot and densitometric analysis of key proteins related to FAO in the kidneys of mice in each group, **P < .01, ***P < .001, n = 4