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. 2020 Mar 17;24(9):5122–5134. doi: 10.1111/jcmm.15153

Figure 6.

Figure 6

Guaiacol suppressed the expression of NFATc1 and osteoclastogenesis‐related genes. A, RANKL induced the nuclear translocation of NFATc1 during osteoclastogenesis, while guaiacol inhibited NFATc1 expression. B, Western blotting of cathepsin K, CTR, MMP‐9 and TRAP, with β‐actin as a reference. 1. RAW264.7 cells; 2. RAW264.7 cells induced with M‐CSF (30 ng/mL), RANKL (50 ng/mL) and PBS; 3. RAW264.7 cells induced with M‐CSF (30 ng/mL) and RANKL (50 ng/mL) and treated with 0.25 μmol/L guaiacol; 4. RAW264.7 cells induced with M‐CSF (30 ng/mL) and RANKL (50 ng/mL) and treated with 0.5 μmol/L guaiacol; 5. RAW264.7 cells induced with M‐CSF (30 ng/mL) and RANKL (50 ng/mL) and treated with 1 μmol/L guaiacol. *P < .05, **P < .01