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. 2019 Sep 30;27(4):1398–1414. doi: 10.1038/s41418-019-0426-2

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Localization of PCD cells in the E11.5 mouse heart. a Frontal section of a transgenic heart at E11.5. Labeled sA5-YFP PCD cells and bodies (white arrows) were found at the trabeculae of both ventricles, in the regions of the developing mitral and tricuspid valves, in the intraventricular septum, and in the moderator band. b–d Magnifications of the boxed areas in a depicting the presence of PCD (sA5-YFP, arrows). Note the striation of cardiomyocytes due to α-actinin staining. Hoechst nuclear signal, sA5-YFP secreted human Annexin V-yellow fluorescent protein, αActinin cardiomyocyte marker, V ventricle, AVC atrioventricular cushion, RA right atrium, LA left atrium, Tr trabeculae, C compact myocardium, MB moderator band (connects the iVS to the anterior papillary muscle), DTV developing tricuspid valve, and DMV developing mitral valve. Bars are 200 µm (a), 10 µm (b, d), and 20 µm (c)